New PDF release: Animals in Scientific Research: An Effective Substitute for

By P. Turner

ISBN-10: 1349064394

ISBN-13: 9781349064397

ISBN-10: 1349064416

ISBN-13: 9781349064410

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Additional info for Animals in Scientific Research: An Effective Substitute for Man?: Proceedings of a Symposium held in April 1982 under the auspices of the Humane Research Trust

Example text

On the one hand there is considerable public concern over the number of chemicals for which inadequate toxicological information exists and to which man is significantly exposed. g. Miller, 1978) . In addition, several hundred new chemicals are being introduced each year which require some form of tOXicological investigation. On the other hand there are mounting economic and moral pressures to change the testing methods that are currently employed. It is pertinent to first consider very briefly in this article some of the strengths and weaknesses of our present in vivo test methods .

E. they no longer require histidine for growth) and are capable of growth on selective medium, which lacks histidine. The number of prototrophic colonies which grow on selective agar plates provides a quantitative measure related to the metagenicity of the compound under test. The agar-overlay test has involved the development of a range of modifications to increase its sensitivity and suitability for the screening of a wide range of environmental chemicals, and these steps are outlined in figure 4 and while the general principles of the test in figure 5.

Ames, B. , McCann, J. and Yamaski, E. (1977) . Methods for detecting carcinogens and mutagens with the Salmonella/mammalianmicrosome mutagenicity test. In Handbook of Mutagenicity Test Procedures, (ed. J. ) Elsevier Scientific Publishing Co. Amsterdam. Arlett, C. F. (1982). The use of cultured mammalian cells in the UKEMS collaborative genotoxicity trial (1981). Mutation Res. 100, 271-276. Bateman, A. J. (1977). The dominant lethal assay in the male mouse. In Handbook of Mutagenicity Test Procedures, (ed.

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Animals in Scientific Research: An Effective Substitute for Man?: Proceedings of a Symposium held in April 1982 under the auspices of the Humane Research Trust by P. Turner

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